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Abstrakt
Effect of Probiotic on Microbiological and Haematological Responsiveness of Cat fish (Heteropnuestes fossilis) Challenged with Bacteria Aeromonas hydrophila and Fungi Aphanomyces invadans
Meeran Mohideen *,Haniffa MA
The use of probiotic for disease prevention and improved nutrition in aquaculture is becoming popular due to an increasing demand for environment friendly aquaculture. Here we used Bacillus subtilis as a probiotic to fish to evaluate the effect of probiotic on microbiological and haematological responsiveness of cat fish (Heteropnuestes fossilis) challenged with bacteria Aeromonas hydrophila and fungi Aphanomyces invadans. Heteropneustes fossilis were collected from local market at Tirunelveli, Tamil Nadu, India. Fish were subjected into microbiological, haematological, physiological observation. In H. fosilis, probiotic accepted fishes gained more weight than that of the control fishes fed with control diet. The gut micro flora of H. fossilis was found to be 6.3×106, 5.7×107, 5.4×105 and 5.1×105 cells in D1 treatment fishes on 104,105,106 and 107 dilutions respectively. The microbiological estimation also showed a dual increase in trial count in T2 injected fish than that of the T3 injected fish. Many factors can influence the immune response of fish. Among them are stressors and environmental factors are natural. In the present investigation behavioural symptoms to pathogenicity such as imbalance, restlessness and avoidance of food were observed. Pathological symptoms include fin necrosis and tail rot which were also observed. In some cases septicaemic ulceration was noticed. Haematological parameters elicited changes which are able to reveal some clues for diagnosis and prognosis of the disease state. T2 fishes were inflicted alterations in TEC, TLC, DLC, and Hb content which indicated decrease state of immunity, when compared with T3 fishes. Bacteria injected fishes showed good healthy status whereas fungal injected fishes showed non healthy status of fish.