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Abstrakt
In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli
Krausova G, Hyrslova I, Jakubec M and Hynstova I
The ability to adhere to intestinal epithelia is a crucial probiotic property since the retention of bacteria in the large intestine is important for probiotic function. Previously, we have shown that the presence of prebiotics decreases the adherence in several prebiotic- probiotic combinations. The objective of this study was to test the effect of three commercially available prebiotics: Orafti GR, Orafti P95, and Orafti Synergy on the adherence of five Lactobacillus strains (Lactobacillus delbrueckii subsp. bulgaricus CCDM 66, Lactobacillus casei subsp. paracasei PE1TB-P, Lactobacillus fermentum RL25, Lactobacillus animalis CCDM 382, and Lactobacillus gasseri PHM-7E1). Adherence was tested using microtiter plates and was evaluated as the percentage of fluorescently labelled bacteria present in the wells after three washes. Adherence was evaluated in both uncoated and mucin-coated polystyrene plates. In accordance with our previous results, the majority of probiotic and prebiotic interactions resulted in at least a 10-fold decrease in adherence. Only one strain, L. gasseri PHM-7E1, exhibited increased adherence to mucin (from 5% to 8% and from 5% to 9%) after the addition of the prebiotics Orafti P95 and GR, respectively. These two combinations appear suitable for further synbiotic testing.