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Abstrakt
Identification of Ɛ-Caprolactam and Melamine in Polyvinyl- Pyrrolidone Powder by Double Injection Micellar Elektrokinetic Chromatography
Ahmad Amini
A double-injection micellar electrokinetic chromatography (DIMEKC) method for the identification of Æ- caprolactam and melamine deliberately added to povidone (polyvinylpyrrolidone) products has been developed. The separations were performed in 89 mM phosphate buffer (pH 7.4) containing 52 mM sodium dodecyl sulfate (SDS) in fused silica capillaries with UV absorption detection at 200 nm. The identification relied on the agreement between the calculated migration time (tmig(c)) of the analytes and the migration time (tmig) of their corresponding reference standards being analysed simultaneously within a double injection run. The migration time of the analytes was calculated from the partial migration times (tmig(p)) as described in this paper. The migration time ratios (tmig(c) / tmig) varied between 0.997 and 1.005 (i.e., 1.001 ± 0.004), indicating good agreement between the observed and calculated migration times.