Einzelzellbiologie

Abstrakt

Glucose-regulated protein 78: A new therapeutic target for amelogenin-induced periodontal tissue regeneration

Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Kensuke Yamamichi, Takaharu Taketomi and Fusanori Nishimura

Amelogenin, the main component of a commercial enamel matrix derivative (Straumann® Emdogain), is widely used in periodontology. It is mainly used in periodontal surgery to stimulate the regeneration of periodontal tissues, including cementum, periodontal ligament and alveolar bone. However, the exact molecular mechanisms underlying amelogenin-induced regeneration are not yet elucidated. Therefore, to gain further insight into how amelogenin induces periodontal tissue regeneration, we performed a protein interaction screen using full-length recombinant amelogenin (rM180) as bait. By coupling affinity chromatography with proteomic analysis, glucose-regulated protein 78 (Grp78) was identified as a novel amelogenin-binding protein. We further demonstrated that the interaction between amelogenin and Grp78 not only contributes to cell proliferation in osteoblastic cells but also promotes cell migration in stem/progenitor cells of the periodontal ligament. Although the enhancing effects of Grp78 in vivo should be further investigated, our findings indicate significant therapeutic potential for amelogenin-induced periodontal tissue regeneration.