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Abstrakt
Expression and Characterization of Salt Stress Induced ABF Transcription Factors from French Bean (Phaseolus Vulgaris)
Prabha C & Nagesh Babu R
ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes. ABFis a bZIP transcription factor and functions in positive modulation of abiotic stress tolerance. It may be an important candidate gene for molecular breeding of stress- tolerant plants. In this study, four ABF coding genes were isolated from French bean(Phaseolus vulgaris) under salt stress (400mM NaCl) conditions.All the four PvABFexhibits complete open reading frame of 1347 bp, encoding a 448 amino acid peptide, and shared high sequence identities with ABFs from other plants. PvABF was sub-cellular targeted to the nucleus, exhibited transactivation activity and could bind to ABF, supporting its role as a transcription factor. Expression levels of PvABFwere induced by treatments with Salt stress conditions.