Einzelzellbiologie

Abstrakt

Effect of lactic acid bacteria on mucosal immune cells against E. coli infection in poultry

Siddique A1*, Rahman S, Ain N and Ullah Khan A

Poultry farming is a well-developed sector of agricultural industry in Pakistan. Extensive use of antibiotics is made in poultry industry to control these pathogens to improve meat and egg production. The present study was conducted to investigate the effect of lactic acid bacteria on immune status against E. coli infection in poultry. Lactobacillus spp. was isolated from a conventional yogurt sample. Lactobacillus spp. was isolated from yogurt. In vivo analysis of Lactobacillus spp. against pathogenic E. coli was performed in a poultry model. Three concentrations maintaining 104, 105 and 106 CFU/ml based on McFarland standard were administered to three groups. In vitro macrophage migration inhibitory factor assay was conducted to check the cellular immune response in poultry against E. coli infection. The results showed that the group administered a high concentration of Lactobacillus spp. had the highest percentage inhibition of macrophages.