Indiziert in
- Öffnen Sie das J-Tor
- Genamics JournalSeek
- Akademische Schlüssel
- JournalTOCs
- CiteFactor
- Ulrichs Zeitschriftenverzeichnis
- Zugang zu globaler Online-Forschung in der Landwirtschaft (AGORA)
- Elektronische Zeitschriftenbibliothek
- Zentrum für Landwirtschaft und Biowissenschaften International (CABI)
- RefSeek
- Verzeichnis der Indexierung von Forschungszeitschriften (DRJI)
- Hamdard-Universität
- EBSCO AZ
- OCLC – WorldCat
- Gelehrtersteer
- SWB Online-Katalog
- Virtuelle Bibliothek für Biologie (vifabio)
- Publons
- Genfer Stiftung für medizinische Ausbildung und Forschung
- Euro-Pub
- Google Scholar
Nützliche Links
Teile diese Seite
Zeitschriftenflyer
Open-Access-Zeitschriften
- Allgemeine Wissenschaft
- Biochemie
- Bioinformatik und Systembiologie
- Chemie
- Genetik und Molekularbiologie
- Immunologie und Mikrobiologie
- Klinische Wissenschaften
- Krankenpflege und Gesundheitsfürsorge
- Landwirtschaft und Aquakultur
- Lebensmittel & Ernährung
- Maschinenbau
- Materialwissenschaften
- Medizinische Wissenschaften
- Neurowissenschaften und Psychologie
- Pharmazeutische Wissenschaften
- Umweltwissenschaften
- Veterinärwissenschaften
- Wirtschaft & Management
Abstrakt
Development of Simple and Rapid Diagnostic Method for Strawberry Latent Ring-spot Virus in Plants Using Loop-Mediated Isothermal Amplification Assay
Jin-Ho Kim, Siwon Lee, Ji-Young Choi, Sue Kyung Kim and Won-Cheoul Jang
Strawberry Latent Ring-spot Virus (SLRSV) is seed or nematodes-transmitted viruses, and causes quantitative and qualitative loss of various crops. SLRSV is a non-reported, potentially control able virus, which is managed at the national level. Currently, RT-PCR and nested PCR system are the standard methods of detecting SLRSV, but more effective methods are required. In this study, loop-mediated isothermal amplification (LAMP) assay was used for detection of SLRSV. As a result, the LAMP assay showed sensitivity similar to that of the currently used method, but is more rapid (approximately 8 hrs), simple and specific. In addition, results can be verified by restriction fragment length polymorphism (RFLP) using BfaI, or sequencing after the LAMP reaction. Therefore, we have shown that the LAMP assay developed in this study is a potential marker for the facilitation of rapid and simple screening of SLRSV in plants, which will ultimately be useful for the diagnosis of SLRSV infected plants and quarantine.