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Abstrakt
Development of a Rapid and Sensitive Routine Method of Analyses for Organochlorine Compounds in Fish: A Metrological Approach
Olivares IRB, Costa SP, Camargo RS and Pacces VHP
The determination of organochlorine compounds in biological samples is very important, since these compounds are lipophilic and persistent in the environment and tend to accumulate in living organisms. However, considering the importance to apply adequate statistical tools to develop and evaluate analytical methods, this paper has as objective the optimization, validation and uncertainty evaluation of a rapid and sensitive routine method for analyses of organochlorine compounds in fish. For optimization of the method, statistical tools such as design of experiments were used, while guidance from SANCO 12571 and Eurachem/Citac Guide were used for the validation of the method and uncertainty estimation of the results respectively. Finally, the method was evaluated using a certified reference material (CRM) in an accredited laboratory that is in accordance with ISO/IEC 17025:2005, completing a suitable metrological approach ensuring that the method is fit for purpose.