Biochemie und analytische Biochemie

Abstrakt

Chitinase-3-Like Protein1 (YKL-40) as Biomarker in Serum of Egyptian Breast Cancer Females

Shaker Olfat G, Nassar Yasser H, Kamel Moataz M, Gad Ziad S and Elantably Ahmed M

Background: YKL-40 is a recently discovered human glycoprotein which is related in amino acid sequence to the chitinase protein family, but has no chitinase activity, its expression was shown to be highly expressed in breast cancer Aim: we aim to detect level of YKL-40 in metastatic and non-metastatic breast cancer patients and comparing the results with fibroadenoma patients and normal females. Methods and materials: 116 female patients were enrolled in this study and they are divided into 37 patients with fibroadenoma, 43 non-metastatic & 36 metastatic patient. Thirty healthy females were also included and served as control. YKL-40 level is detected in serum of studied groups using ELISA kit provided from Quantikine R&D systems, Minneapolis, USA. Results: There is statistical significant difference regarding YKL-40 level between the control group versus nonmetastatic group as well as metastatic group, and between fibroadenoma group and non-metastatic as well as metastatic breast cancer group (P<.001 each). Also there is high statistical significant difference between N1, N2 and N3 in non-metastatic group as well as between N2 and N3 in metastatic group (each<.001). Conclusion: We concluded that there is significantly elevated serum YKL-40 level in breast carcinoma compared to women with benign breast tumors and healthy controls.