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Abstrakt
Bacillus subtilis A9 PRODUCING LIPASE ISOLATED FROM SHRIMP PASTE
Yati Sudaryati Soeka ,Maman Rahmansyah
Lipase-producing-bacterium had been isolated from “Terasi (Shrimp Paste)”, a traditional fermented food made from penaeid prawn. The isolates cultures produce holozone on Bypta Agar Culture because of its enzymatic activity. By using 16S rRNA amplification within the genomic DNA, the isolate identified as Bacillus subtilis A9. The isolate become collection belong to Indonesia Culture Collection (InaCC), Research Center for Biology, Indonesian Institute of Sciences. Furthermore, crude enzyme extracted from isolates then tested to catalyze algal oil, and it turn into various fatty acids by using precipitated (PEM) and immobilized (IEM) crude enzyme. PEM and IEM reach optimum activity at 96 hours to change olive oil as standard substance. Ten fatty acids were achieved as due to crude enzymes esterification. The highest percentage of lauroleic acid was obtained, and followed by palmitic and stearic acid. The IEM was able to produce behenic acid in 13.6 percent quantity