Assessment of Blighia sapida on Cholinergic and Antioxidant Enzymes; Possible use of the Plant Stem-Bark Extract as a Biological Pest Controlling Agent

Adekola Mukaila Babatunde1*, Taiwo Adewale Mathew1, Oriyomi Vincent Olumayowa2, Ogunleye Olalekan Seyi1, Oyebamiji Adeola Tope1, Raheem Abdul Rasheed Omotola1, Kasali Sodiq Oluwafemi1, Bello Iyabo Raliat1

Reduction of cholinergic and metabolizing enzymes by natural products are safer pest-controlling alternatives. The study investigated the ability of Blighia sapida stem-bark extract relative to Rambo, a synthetic pesticide, to interfere with Acetylcholinesterase (AChE), Glutathione S-Transferase (GST), and biomarkers in the brain, liver, and blood of Wistar rat. Rat brain and liver were excised and blood was collected into heparinized tubes at the end of a 28- day experiment for biochemical investigations. The activities of AChE and GST decreased at a dose-dependent rate (P<0.05). A non-significant difference in Alkaline Phosphatase (ALP) for all the treated groups and a dose-dependent increase in total protein concentration was detected. The extract did not significantly alter Alanine Transaminase (ALT), Aspartate Transaminase (AST), and ALP, particularly at repeated doses of 50 mg/kg and 100 mg/kg. The extract of Blighia sapida reduced AChE and GST activity; a property that could be exploited in the formulation of pest control agents.